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氟苯尼考ELISA快速检测试剂盒(检蜂蜜/组织,灵敏度0.5ppb,两步法)

检测试剂盒12.jpg

氟苯尼考ELISA快速检测试剂盒

 

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物氟苯尼考将和微孔条上预包被的偶联抗原竞争抗氟苯尼考抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物氟苯尼考的含量成负相关,与标准曲线比较即可得出相应残留物氟苯尼考的含量。

 

 

二、试剂盒特性

u  试剂盒规格     96/

u  试剂盒灵敏度:   0.5ppb

u  试剂盒检测范围: 0.540.5ppb;

u  试剂盒贮存条件:  28

u  板内变异系数:   <5%

u  板间变异系数:   <15%;

u  孵育温度:       25;

u  孵育时间:       60min-30min-30min

u  样本检测下限:

蜂蜜、组织······································  0.5ppb

 

u  交叉反应率

氟苯尼考··································· 100%

甲砜霉素   ································ < 0.1%

氟苯尼考胺  ·································11%

氯霉素  ·········································< 0.1%

 

u  样本回收率

   ······································  80%±18%

   ······································  70%±14%