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莱克多巴胺(Ract)ELISA检测试剂盒(检组织/尿样,灵敏度0.05ppb,一步法)

检测试剂盒12.jpg

莱克多巴胺(Ract)ELISA快速检测试剂盒

 

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物莱克多巴胺将和微孔条上预包被的偶联抗原竞争抗莱克多巴胺抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物莱克多巴胺的含量成负相关,与标准曲线比较即可得出相应残留物莱克多巴胺的含量。

 

二、试剂盒特性

试剂盒规格 :        96/

试剂盒灵敏度:    0.05ppb

曲线范围:            0.0540.5ppb

孵育温度:          25℃

孵育时间:          30min15min

试剂盒贮存条件:  28℃

板内变异系数:     <5%

板间变异系数:     <15%;

 

样本检测下限

          ················································· 0.2ppb

尿    样      ················································ 0.1ppb

 

u  交叉反应率

莱克多巴胺(Ractopamine)  ···················100%

多巴酚丁胺       ···············································< 1%

沙丁胺醇(Salbutamol ) ··························<0.1%

克伦特罗(Clenbuterol·····························<0.1%


u  样本回收率

组织、尿样    ··············································· 60%~120%